Related Experiment Video
Updated: Aug 19, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Identification of more sequence variations in the D8S1179 locus
Sandra Hering1, Ralf Nixdorf, Jan Dressler
1Institute of Legal Medicine, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. sandra.hering@mailbox.tu-dresden.de
Abstract:
Routine STR-typing of 10,293 buccal swabs using different multiplex kits presented discordant D8S1179 profiles in four cases. Sequencing analysis identified a G-to-A transition upstream to the repeat, and an A-to-T transversion and a G-to-A transition downstream to the repeat. In the fourth case a four-base pair deletion downstream resulted in altered genotypes using different primer pairs. Current searching algorithms of the German DNA database are not capable of matching profiles that are divergent in only one STR-locus. Thus, to accommodate matching requirements and to avoid errors in individual genetic characterization for D8S1179, as described here, it is suggested that alternative primer pairs be used for routine genotyping as a matter of course.
Related Concept Videos
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Single Nucleotide Polymorphisms-SNPs
Modern Molecular Taxonomy

