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[The study on HLA-Cw polymorphism from Xi'an Han population by PCR-sequence specific oligonucleotide probe]
Hong-bo Zhang1, Jiang-hua Lai, Jun-hai Zhao
1The National Laboratory of the Ministry of Health for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China. zhanghb@mail.xjtu.edu.cn
Objective:
To determine the HLA-Cw allele and genotype frequencies from Xi'an Han population and obtain genetic data.
Methods:
The results of HLA-Cw typing for 130 randomly selected from Xi'an Han population were obtained by using the PCR-Sequence Specific Oligonucleotide Probes (SSOP).
Results:
In this investigation, 16 alleles were detected among 130 unrelated individuals with frequencies from 0.0077 to 0.1588 and HLA-Cw*01,03,07 were the most common HLA-Cw alleles. We have made a survey of HLA-Cw alleles frequencies in Xi'an Han Population, with blank frequency being lowered to 0.018 2.
Conclusion:
The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by Hi-square test. The frequency data can be used in forensic and paternity tests, transplant matching and anthropology.