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Related Experiment Videos

Vancomycin-enriched corneal storage medium.

T L Steinemann1, H E Kaufman, R W Beuerman

  • 1Lions Eye Research Laboratories, LSU Eye Center, New Orleans 70112.

American Journal of Ophthalmology
|May 15, 1992
PubMed
Summary
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Vancomycin is stable in corneal storage solutions and does not harm endothelial cells. This antibiotic can potentially supplement gentamicin to prevent endophthalmitis after corneal transplants.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Transplantation Science

Background:

  • Antibiotics in corneal preservation solutions are crucial for preventing endophthalmitis post-transplant.
  • Gentamicin is currently used at high concentrations, necessitating investigation into supplementary antibiotics.
  • Vancomycin's stability and endothelial cell toxicity in corneal storage media were previously undocumented.

Purpose of the Study:

  • To evaluate the stability of vancomycin in corneal storage media.
  • To assess the in vitro toxicity of vancomycin on donor corneal endothelial cells.
  • To determine the potential of vancomycin as an adjunct to gentamicin for preventing post-keratoplasty endophthalmitis.

Main Methods:

  • Vancomycin (100 µg/ml) was added to two corneal storage media containing gentamicin.

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  • Antibiotic stability was monitored over five months at neutral pH (7.2).
  • Donor corneal tissue was stored for two weeks at 4°C, and endothelial cell morphology was analyzed.

    • Main Results:

    • Vancomycin remained stable in solution, with concentrations exceeding 90 µg/ml for the initial five weeks.
    • No significant differences in endothelial cell shape, borders, swelling, or apical holes were observed between vancomycin-treated and control groups.
    • The stability and lack of endothelial toxicity support vancomycin's potential use.

    Conclusions:

    • Vancomycin is stable in corneal storage media at neutral pH for extended periods.
    • Vancomycin does not exhibit in vitro toxicity to corneal endothelial cells.
    • Vancomycin shows promise as a supplementary antibiotic to gentamicin for preventing endophthalmitis in corneal transplantation.