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A density method for determining plasma and red blood cell volume.

J S Lee1, L P Lee

  • 1Department of Biomedical Engineering, University of Virginia, Charlottesville.

Annals of Biomedical Engineering
|January 1, 1992
PubMed
Summary

Researchers used a sensitive density system to measure blood and plasma density changes in rabbits after saline infusion. This allowed calculation of red blood cell and plasma volumes, yielding a Fcell ratio of 0.82 +/- 0.02.

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Area of Science:

  • Physiology
  • Biomedical Engineering

Background:

  • Accurate measurement of blood and plasma volumes is crucial in physiological studies.
  • The Fåhraeus effect influences red blood cell distribution in microvasculature, impacting density measurements.

Purpose of the Study:

  • To develop a non-invasive method for determining red blood cell volume and plasma volume.
  • To validate a vasculature model incorporating the Fåhraeus effect for volume calculations.
  • To assess the ratio of calculated hematocrit to arterial hematocrit (Fcell).

Main Methods:

  • Utilized a highly sensitive density measuring system to monitor blood and plasma density changes.
  • Infused a saline bolus into the rabbit circulation to induce measurable density variations.
  • Developed and applied a set of equations derived from a vasculature model, including the Fåhraeus effect, to calculate volumes from density data.

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Main Results:

  • Successfully measured decreases in blood and plasma density following saline bolus infusion.
  • Calculated red blood cell volume and plasma volume based on density changes and the vasculature model.
  • Determined a Fcell value of 0.82 +/- 0.02, representing the ratio of calculated to arterial hematocrit.

Conclusions:

  • The density measuring system and derived equations provide a viable method for estimating red blood cell and plasma volumes.
  • The Fåhraeus effect is a significant consideration in vasculature models for accurate volume calculations.
  • The obtained Fcell value offers insights into hematocrit distribution and measurement accuracy in vivo.