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Amniotic fluid components and changes due to storage conditions
J L Zaidman1, M Waron, S Meyer
1Division of Laboratories, Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, Israel.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|January 1, 1992
Summary
Amniotic fluid composition changes with storage temperature. Enzymes like creatine kinase decreased significantly at -20°C, but were more stable at -70°C, impacting analyte analysis.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Reproductive Medicine
Background:
- Amniotic fluid analysis is crucial for assessing fetal well-being.
- Standardized storage conditions are essential for accurate biochemical testing.
- Variability in amniotic fluid composition can be influenced by storage and physiological factors.
Purpose of the Study:
- To investigate the impact of different storage temperatures on amniotic fluid analyte stability.
- To determine optimal storage conditions for various amniotic fluid analytes.
- To understand the variability of amniotic fluid analytes during gestation.
Main Methods:
- Amniotic fluid samples were stored at -20°C and -70°C.
- Concentrations of specific analytes, including enzymes (creatine kinase, lactate dehydrogenase) and electrolytes (Na, K, Ca, Cl), were measured.
- Inter-individual variability was assessed.
Main Results:
- Catalytic concentrations of creatine kinase and lactate dehydrogenase decreased significantly after storage at -20°C.
- Enzyme decreases were less pronounced at -70°C.
- Electrolytes (Na, K, Ca, Cl) exhibited low inter-individual variability, suggesting homeostatic control.
- Enzyme activities showed high variability, potentially due to gestational changes or fetal conditions.
Conclusions:
- Optimal storage conditions for amniotic fluid samples vary depending on the specific analyte being measured.
- Enzyme stability is temperature-dependent, with -70°C being superior to -20°C.
- Electrolyte concentrations appear stable and homeostatically controlled during gestation, while enzyme activities are more dynamic and susceptible to various factors.