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Related Experiment Videos

Amniotic fluid components and changes due to storage conditions.

J L Zaidman1, M Waron, S Meyer

  • 1Division of Laboratories, Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, Israel.

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|January 1, 1992
PubMed
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Amniotic fluid composition changes with storage temperature. Enzymes like creatine kinase decreased significantly at -20°C, but were more stable at -70°C, impacting analyte analysis.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Reproductive Medicine

Background:

  • Amniotic fluid analysis is crucial for assessing fetal well-being.
  • Standardized storage conditions are essential for accurate biochemical testing.
  • Variability in amniotic fluid composition can be influenced by storage and physiological factors.

Purpose of the Study:

  • To investigate the impact of different storage temperatures on amniotic fluid analyte stability.
  • To determine optimal storage conditions for various amniotic fluid analytes.
  • To understand the variability of amniotic fluid analytes during gestation.

Main Methods:

  • Amniotic fluid samples were stored at -20°C and -70°C.
  • Concentrations of specific analytes, including enzymes (creatine kinase, lactate dehydrogenase) and electrolytes (Na, K, Ca, Cl), were measured.

Related Experiment Videos

  • Inter-individual variability was assessed.

    • Main Results:

    • Catalytic concentrations of creatine kinase and lactate dehydrogenase decreased significantly after storage at -20°C.
    • Enzyme decreases were less pronounced at -70°C.
    • Electrolytes (Na, K, Ca, Cl) exhibited low inter-individual variability, suggesting homeostatic control.
    • Enzyme activities showed high variability, potentially due to gestational changes or fetal conditions.

    Conclusions:

    • Optimal storage conditions for amniotic fluid samples vary depending on the specific analyte being measured.
    • Enzyme stability is temperature-dependent, with -70°C being superior to -20°C.
    • Electrolyte concentrations appear stable and homeostatically controlled during gestation, while enzyme activities are more dynamic and susceptible to various factors.