Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monolithic bioreactor immobilizing trypsin for high-throughput analysis.

Masaru Kato1, Kenji Inuzuka, Kumiko Sakai-Kato

  • 1Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada Shizuoka, Shizuoka, 422-8526, Japan.

Analytical Chemistry
|March 15, 2005
PubMed
Summary

This study developed a miniaturized trypsin reactor using a silica monolith for efficient protein digestion. The immobilized enzyme shows high activity, stability, and reusability in a 96-well plate format.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New-onset Systemic Lupus Erythematosus after Methimazole Exposure Followed by Autoantibody Emergence.

Internal medicine (Tokyo, Japan)·2026
Same author

Low-dose, not low-risk: potential adrenal suppression identified by a risk-based approach to ACTH testing.

Clinical rheumatology·2026
Same author

JCS/JPCPHS 2025 Guideline on Pulmonary Hypertension and Pulmonary Embolism/Deep Vein Thrombosis.

Circulation journal : official journal of the Japanese Circulation Society·2026
Same author

Deletion of Tgf-β1 From CD206<sup>+</sup> M2 Macrophages Ameliorates Obesity-Induced Suppression of Myogenesis and AMPK Phosphorylation in Skeletal Muscle.

Journal of cachexia, sarcopenia and muscle·2026
Same author

Lansoprazole-Induced Microscopic Colitis Mimicking Anticancer Drug-Related Diarrhea During Lenvatinib and Pembrolizumab Combination Therapy: A Case Report.

Clinical case reports·2026
Same author

A Rare Case of Bartter Syndrome Type 3 Diagnosed in Elderly Age.

Case reports in endocrinology·2026

Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Enzyme immobilization is crucial for developing reusable biocatalysts.
  • Miniaturized reactors offer advantages in sample throughput and reagent consumption.
  • Trypsin is a key enzyme for protein digestion in proteomics.

Purpose of the Study:

  • To develop a miniaturized, on-plate trypsin reactor using a sol-gel encapsulated enzyme on a silica monolith.
  • To evaluate the activity, stability, and reusability of the immobilized trypsin.
  • To assess the reactor's applicability for protein digestion in a 96-well microtiter plate format.

Main Methods:

  • Fabrication of a porous silica monolith.
  • Encapsulation of trypsin into a gel matrix using the sol-gel method.

Related Experiment Videos

  • Immobilization of the trypsin-gel onto the silica monolith.
  • Optimization of sol-gel reaction for thin film formation.
  • Activity and kinetic analysis of the immobilized trypsin.
  • Testing durability, repeatability, and protein digestion capability.

    • Main Results:

    • Successful encapsulation of active trypsin into a gel matrix on a silica monolith.
    • High catalytic turnover rate and absence of diffusional limitations for immobilized trypsin.
    • Satisfactory durability and repeatability of the trypsin-coated monolith.
    • Enhanced stability of encapsulated trypsin compared to free solution.
    • Demonstrated applicability for on-plate protein digestion.

    Conclusions:

    • The developed miniaturized trypsin reactor is efficient and stable.
    • The sol-gel encapsulation on a silica monolith provides a robust platform for enzyme immobilization.
    • This on-plate bioreactor system is suitable for high-throughput protein digestion applications.