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Updated: Aug 19, 2026
11:39
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
Published on: September 21, 2012
An improved formulation of the zirconyl hematoxylin stain for acidic mucins
1Barry University School of Graduate Medical Sciences, Miami Shores, Florida 33161, USA.
Abstract:
Zirconyl hematoxylin stains acidic mucins darkly and specifically using a solution of 100 mg hematoxylin, 5 ml ethanol, 5 ml 0.5% sodium iodate, 400 mg zirconyl chloride octahydrate, and 30 ml 25% aqueous glycerol. The stain is especially advantageous for studying goblet cells and Paget cells.
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