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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
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Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays

Mong-Hsun Tsai1, Hailing Yan, Xi Chen

  • 1Radiation Oncology Sciences Program, Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 8717 Grovemont Cr., Room 109J, Gaithersburg, MD 20878, USA.

Molecular Biotechnology
|March 16, 2005
PubMed

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...