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Updated: Aug 19, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

A single-stage polymerase-based protocol for the introduction of deletions and insertions without subcloning

John C Salerno1, Rachel J Jones, Eda Erdogan

  • 1Biology Department, Rensselaer Polytechnic Institute, Science Center, 1W14, 110 8th St., Troy, NY 12180, USA. salers2@rpi.edu

Molecular Biotechnology
|March 16, 2005
PubMed

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