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[A case-control study on the relationship between stroke and plasma homocysteine level and the mutation of MTHFR
Hui Ye1, Jiang-tao Yan, Jiao-mei Shao
1Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Insights
Elevated plasma homocysteine (Hcy) levels are linked to stroke in Chinese populations. However, the methylenetetrahydrofolate reductase (MTHFR) C677T gene mutation showed no association with Hcy levels or stroke risk.
Area of Science:
- Biochemistry
- Genetics
- Neurology
Context:
- Stroke is a leading cause of death and disability globally.
- Elevated plasma homocysteine (Hcy) is a potential risk factor for cardiovascular and cerebrovascular diseases.
- The methylenetetrahydrofolate reductase (MTHFR) gene plays a role in Hcy metabolism.
Purpose:
- To investigate the association between plasma Hcy levels, MTHFR C677T gene mutation, and stroke in a Chinese population.
- To determine if MTHFR C677T mutation influences Hcy levels or stroke risk.
Summary:
- This study included 300 stroke patients and 300 controls, measuring plasma Hcy levels and MTHFR C677T mutation status.
- Results indicated significantly higher plasma Hcy levels in the stroke group compared to controls.
- No significant difference in MTHFR C677T mutation rates was found between groups, nor did the mutation affect Hcy levels.
Impact:
- Increased plasma Hcy levels are implicated in stroke occurrence among Chinese individuals.
- The MTHFR C677T mutation was not found to be associated with Hcy levels or stroke.
- Plasma Hcy level management may be crucial for preventing cardio-cerebro-vascular diseases in this population.
Objective:
To study the relationship of stroke and plasma homocysteine (Hcy) level and the mutation of methylenetetrahydrofolate reductase (MTHFR) gene.
Methods:
Three hundred patients with stroke were included as the case group in this study while 300 subjects without stroke matched with the case group for sex and age were collected as the control group. Plasma Hcy level was measured using HPLC and MTHFR 667C-->T mutant was determined by polymerase chain reaction and restriction fragment analysis. The relationship of stroke and plasma Hcy level and the mutation of MTHFR gene was then analysed.
Results:
The plasma Hcy level of the case group was significantly higher than that of the control group (16.92 +/- 3.43 micromol/L vs. 14.57 +/- 2.59 micromol/L, P <0.05). There was no significant difference in mutation rate of MTHFR C677T between stroke group and control group (P >0.05), as well as between ischemic stroke group and homorrhagic stroke group (P >0.05). The mutation of MTHFR C677T had no significant influence on the Hcy level (15.28 +/- 2.17 micromol/L vs. 15.11 +/- 3.81 micromol/L, P >0.05).
Conclusion:
Increased plasma Hcy level played an important role in the occurrence of stroke in Chinese people, and the mutation of MTHFR C677T was not associated with Hcy level or stroke which led to the speculation that plasma Hcy level was important in the prevention of cardio-cerebro-vascular diseases among Chinese people.
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