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Extended-range glucose biosensor via layer-by-layer assembly incorporating gold nanoparticles.
Wei Zhao1, Jing-Juan Xu, Hong-Yuan Chen
1Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
Frontiers in Bioscience : a Journal and Virtual Library
|March 17, 2005
Summary
We developed efficient glucose biosensors using multilayer films containing glucose oxidase (GOx) and gold nanoparticles (AuNP). These biosensors offer a wide detection range and good stability for glucose sensing applications.
Area of Science:
- Materials Science
- Nanotechnology
- Biosensors
Background:
- Glucose oxidase (GOx) is crucial for glucose detection.
- Gold nanoparticles (AuNP) can enhance biosensor performance.
- Layer-by-layer self-assembly is a versatile film fabrication technique.
Purpose of the Study:
- To develop efficient glucose biosensors using GOx/polyelectrolyte/AuNP multilayer films.
- To investigate the role of AuNPs in enhancing biosensor properties.
- To optimize biosensor performance by controlling film composition and structure.
Main Methods:
- Layer-by-layer self-assembly for multilayer film fabrication.
- Electrochemical impedance spectroscopy (EIS) and UV-visible spectroscopy for film characterization.
- Fabrication of a biosensor using poly(dimethyldiallylammonium chloride) (PDDA)/AuNP/PDDA/GOx multilayer films.
Main Results:
- Multilayer films demonstrated regular growth.
- AuNPs increased GOx loading and activity, improving electron transfer.
- The biosensor showed a linear calibration range of 0–60.0 mM for glucose detection.
- A low detection limit of 3.0 microM and an extended dynamic range up to 120 mM were achieved.
- The biosensor exhibited good stability and reproducibility.
Conclusions:
- GOx/PE/AuNP multilayer films are effective for glucose biosensing.
- Controlling AuNP loading and layer number allows for tailored biosensor performance.
- The developed biosensor offers a promising platform for accurate and reliable glucose monitoring.