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09:14
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Published on: June 13, 2014
Stable expression of sialyl-Tn antigen in T47-D cells induces a decrease of cell adhesion and an increase of cell
Sylvain Julien1, Chann Lagadec, Marie-Ange Krzewinski-Recchi
1Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS no. 8576, GDR CNRS no. 2590, France.
Breast Cancer Research and Treatment
|March 17, 2005
Summary
Sialyl-Tn antigen expression, induced by ST6GalNAc I, alters breast cancer cell behavior. This modification decreases cell growth and adhesion while increasing migration, offering insights into cancer development.
Area of Science:
- Glycobiology
- Cancer Biology
- Molecular Oncology
Background:
- Sialyl-Tn antigen is overexpressed in epithelial cancers, correlating with poor prognosis.
- Its synthesis is catalyzed by CMP-Neu5Ac:GalNAc alpha2,6-sialyltransferase (ST6GalNAc I), leading to shortened O-glycan chains.
- Typical breast cancer cell lines lack ST6GalNAc I and sialyl-Tn expression.
Purpose of the Study:
- To generate and characterize T47-D breast cancer cell clones expressing the sialyl-Tn antigen.
- To investigate the impact of sialyl-Tn expression on cancer cell biological features.
- To explore the role of altered O-glycosylation in breast cancer progression.
Main Methods:
- Stable transfection of T47-D cells with human ST6GalNAc I cDNA.
- Analysis of sialyl-Tn antigen expression on cell surface glycoproteins, including MUC1.
- Assessment of cell growth, adhesion, and migration in transfected vs. mock-transfected clones.
Main Results:
- Generated T47-D clones stably expressing sialyl-Tn antigen.
- Sialyl-Tn expression was observed on high molecular weight O-glycoproteins like MUC1.
- Sialyl-Tn positive clones exhibited decreased cell growth and adhesion, alongside increased cell migration.
Conclusions:
- Altering the O-glycan pattern is sufficient to modify cancer cell biological features.
- Sialyl-Tn expression significantly impacts breast cancer cell behavior.
- These T47-D clones provide a model for further in vivo studies on O-glycosylation in breast cancer.
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