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Updated: Aug 19, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
[Clinicopathologic significance of CD105 mRNA expression in human breast carcinoma]
Da Pang1, Feng Liu, Ying-Wei Xue
1Department of Abdominal Surgery, The Third Affiliated Hospital Of Harbin Medical University, Harbin 150040, China. hljpangda@sina.vip.com
Objective:
To study endoglin (CD105) gene expression in breast cancer and its clinicopathologic significance.
Methods:
In 40 patients with breast cancers, CD105 mRNA was detected at center and periphery of tumor and at nearby normal tissue by RT-PCR.
Results:
The difference in CD105 mRNA expressions between cancer and normal breast tissue was significant (t = 12.08, P < 0.05), and the expression was significantly higher at the tumor periphery than at the tumor center (t = 7.52, P < 0.05). CD105 over-expression was related to lymph node metastases (t = 2.71, P < 0.05), but not to age, tumor size, pathologic grade or pathologic type (P > 0.05).
Conclusion:
CD105 over-expression may play a crucial role in the progression of breast cancer and lymph node metastasis.