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Updated: Aug 19, 2026

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Published on: April 17, 2017
Isolation of isomers based on hydrogen/deuterium exchange in the gas phase
Ulf Mazurek1, Melinda A McFarland, Alan G Marshall
1Department of Physical Chemistry and The Farkas Center for Light Induced Processes, The Hebrew University of Jerusalem, Giv'at Ram, Jerusalem 91904, Israel. ykv@chem.ch.huji.ac.il
Abstract:
A method to isolate isomers in a Fourier transform ion cyclotron resonance mass spectrometer on the basis of their different hydrogen/deuterium exchange rates has been developed and applied to the protonated serine octamer cluster. Isolation allows interrogation of each isomer by infrared multi-photon dissociation to determine any differences in the building blocks that form these clusters. The experimental results strongly support previous assignments of an all-zwitterion structure to the slower-exchanging isomer and an all-neutral structure to its faster- exchanging counterpart.
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