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Published on: August 16, 2013
C-terminus of p47(phox) is required for interaction with leukocyte type rat 12-lipoxygenase
Michiharu Tanabe1, Hideki Kamitani, Takashi Watanabe
1Department of Medical Biochemistry and Biophysics, Karolinska Instituet, Stockholm, Sweden.
Abstract:
In yeast two-hybrid system, rat 12-lipoxygenase (12-LO) bound to complete (390 amino acids) or the N-terminus truncated form of human p47 (phox), but not to the C-terminus truncated form (residues 1-286). When glutathione S-transferase fused human p47(phox) was added to an in vitro 12-LO enzyme activity assay, formation of 12-hydroperoxyeicosatetraenoic acid was reduced significantly compared to the C-terminus truncated form. These results indicate that C-terminus of p47(phox) is important for its interaction to rat 12-LO.
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