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Updated: Aug 19, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Ribotyping of Burkholderia mallei isolates
Steven P Harvey1, Jennifer M Minter
1US Army Edgewood Chemical Biological Center, 5138 Blackhawk Road, Aberdeen Proving Ground, MD 21010-5424, USA. steve.harvey@us.army.mil
Abstract:
In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world. DNA samples from each isolate were digested separately with PstI and EcoRI enzymes and probed with an Escherichia coli-derived 18-mer rDNA sequence to identify diagnostic fragments. Seventeen distinct ribotypes were identified from the combined data obtained with the two restriction enzymes. The results demonstrate the general utility of ribotyping for the subspecies identification of B. mallei isolates.
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