Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altering protein specificity: techniques and applications.

Nina M Antikainen1, Stephen F Martin

  • 1Department of Chemistry and Biochemistry, The Institute of Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA.

Bioorganic & Medicinal Chemistry
|March 23, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Sigma‑2 Receptor/Transmembrane Protein 97 in Pain Modulation: Tracing Historical Roots and Current Research Directions".

ACS pharmacology & translational science·2025
Same author

Transmembrane Protein 97 (TMEM97): Molecular Target and Treatment in Age-Related Macular Degeneration (AMD).

Biomolecules·2025
Same author

Total Synthesis of (+)-Pierisketone B.

Journal of the American Chemical Society·2025
Same author

Sigma‑2 Receptor/Transmembrane Protein 97 in Pain Modulation: Tracing Historical Roots and Current Research Directions.

ACS pharmacology & translational science·2025
Same author

Delivery of a novel neuroprotective compound to the retina in rat and rabbit animal models.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Loss of Sigma-2 Receptor/TMEM97 Is Associated with Neuropathic Injury-Induced Depression-Like Behaviors in Female Mice.

eNeuro·2024
Same journal

Transport specificity of FpvA and FpvB for pyoverdine-antibiotic conjugates in Pseudomonas aeruginosa.

Bioorganic & medicinal chemistry·2026
Same journal

Design and engineering of μO-conotoxin MfVIA mutants to enhance Na<sub>V</sub>1.8 inhibition and analgesic efficacy in inflammatory pain.

Bioorganic & medicinal chemistry·2026
Same journal

Recent advances in Camptothecin-derived antibody-drug conjugates.

Bioorganic & medicinal chemistry·2026
Same journal

CDK4/6-targeted therapy: From clinical inhibitors to emerging strategies to overcome resistance.

Bioorganic & medicinal chemistry·2026
Same journal

Coumarin-sulfonamide hybrids as PKM2 activators induce metabolic reprogramming and suppress ovarian cancer cell growth.

Bioorganic & medicinal chemistry·2026
Same journal

Recent advances in the development of small-molecule drugs based on covalent reversible inhibitors.

Bioorganic & medicinal chemistry·2026
See all related articles

Protein engineering creates novel proteins for selective chemical and biological transformations. Methods like mutation, evolution, and screening yield new enzymes for diverse applications and research.

Area of Science:

  • Biochemistry and Molecular Biology
  • Biotechnology
  • Enzyme Engineering

Background:

  • Protein engineering enables the creation of novel proteins with specific catalytic functions.
  • Enzymes are crucial catalysts for chemical and biological transformations.
  • Altering enzyme specificity is key for industrial and medicinal applications.

Purpose of the Study:

  • To provide a critical overview of methods for creating new functional proteins with altered specificity.
  • To highlight practical case studies of protein engineering applications.
  • To discuss the role of engineered proteins in synthetic chemistry, medicinal chemistry, and biological pathway elucidation.

Main Methods:

  • Rational and random mutagenesis for altering enzyme substrate specificity.

Related Experiment Videos

  • Evolutionary techniques including selection and screening for identifying desired protein variants.
  • Comparative analysis of different protein engineering strategies.

    • Main Results:

    • Successful generation of novel proteins with modified specificity profiles.
    • Demonstration of engineered proteins in solving complex problems in chemistry and biology.
    • Insights into structure-function relationships through protein engineering.

    Conclusions:

    • Protein engineering is a powerful approach for designing enzymes with tailored functions.
    • Engineered proteins have significant potential in synthetic chemistry, drug discovery, and fundamental biological research.
    • The discussed methods offer versatile tools for advancing protein science and its applications.