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Chromatographic media for bioseparation.

Alois Jungbauer1

  • 1Department of Biotechnology, University of Natural Resources and Applied Life Science, A-1190 Vienna, Austria. alois.jungbauer@boku.ac.at

Journal of Chromatography. A
|March 24, 2005
PubMed
Summary

This review explores chromatography media for bioseparation, highlighting large-pore materials and convective mass transport in monoliths for efficient separation of large molecules and nanoparticles. Fast operation is a key feature of modern chromatography.

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Area of Science:

  • Bioseparation engineering
  • Chromatography science
  • Materials science

Background:

  • Chromatographic steps are central to bioseparation processes, including primary recovery.
  • Fluidized beds are sometimes used for chromatographic separation instead of fixed beds.

Purpose of the Study:

  • To describe the action principles and features of chromatography media.
  • To categorize different chromatography media based on physical and chemical properties.

Main Methods:

  • Review of action principles of chromatography media.
  • Analysis of physical and chemical properties of media.
  • Categorization of media for bioseparation.

Main Results:

  • Bioseparation media are characterized by large pores and particle sizes.

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  • Monoliths are ideal for ultralarge molecules (e.g., plasmids) and nanoparticles (e.g., viruses) due to convective mass transport.
  • Modern chromatography media enable fast operations, with residence times as low as 3 minutes.

    • Conclusions:

    • Chromatography media selection depends on molecule size and desired transport mechanisms.
    • Monolithic media offer advantages for large biomolecules and nanoparticles.
    • Advancements in chromatography media facilitate rapid and efficient bioseparation.