Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity processes realized on high-flow-through methacrylate-based macroporous monoliths.

Galina A Platonova1, Tatiana B Tennikova

  • 1Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg 199 004, Russia.

Journal of Chromatography. A
|March 24, 2005
PubMed
Summary

Rigid macroporous polymers enable rapid bio-separation and synthesis. These monolithic materials offer fast affinity separation and efficient biocatalysis, advancing analytical and preparative applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants.

ACS physical chemistry Au·2024
Same author

Non-natural 2<i>H</i>-azirine-2-carboxylic acids: an expedient synthesis and antimicrobial activity.

RSC advances·2022
Same author

Insertion of metal carbenes into the anilinic N-H bond of unprotected aminobenzenesulfonamides delivers low nanomolar inhibitors of human carbonic anhydrase IX and XII isoforms.

European journal of medicinal chemistry·2021
Same author

Arene-Ruthenium(II) Complexes Containing 11<i>H</i>-Indeno[1,2-<i>b</i>]quinoxalin-11-one Derivatives and Tryptanthrin-6-oxime: Synthesis, Characterization, Cytotoxicity, and Catalytic Transfer Hydrogenation of Aryl Ketones.

ACS omega·2020
Same author

Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs.

Polymers·2020
Same author

Functionalized Pt(II) and Ir(III) NIR Emitters and Their Covalent Conjugates with Polymer-Based Nanocarriers.

Bioconjugate chemistry·2020

Area of Science:

  • Polymer Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Rigid macroporous polymers, developed in the late 1980s, have established a significant scientific and practical field.
  • Monolithic stationary phases are integral to bioseparation (chromatography) and bioconversion (enzyme reactors).

Purpose of the Study:

  • To highlight the versatility of rigid macroporous polymers in various scientific and practical applications.
  • To demonstrate the speed and efficiency of bioaffinity separation using these materials.

Main Methods:

  • Utilizing immobilized bioaffinity ligands on rigid macroporous polymer supports.
  • Developing dynamic methods for rapid analytical and preparative separations.
  • Applying monoliths in enzyme reactors and solid-phase synthesis.

Related Experiment Videos

Main Results:

  • Demonstrated the first instance of rapid bioaffinity pairing and affinity separation within seconds.
  • Showcased improved kinetics in biocatalyzed reactions due to minimal sorbent surface influence.
  • Presented applications in solid-phase chemical synthesis of peptides and oligonucleotides.

Conclusions:

  • Rigid macroporous polymers are powerful tools for rapid bioseparation and synthesis.
  • Bioaffinity modes on these supports offer promising analytical and preparative isolation capabilities.
  • The technology facilitates efficient enzyme reactors and solid-phase synthesis of biomacromolecules.