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Qualitative microanalysis of ions and ultrastructural changes in dentin exposed to laser irradiation and to metal
Carlos E Glauche1, Patricia M de Freitas, Nilson D Vieira
1Professional Master Course of Lasers in Dentistry, Institute of Energy and Nuclear Research, IPEN, São Paulo, SP 05508-900, Brazil.
Background And Objectives:
This study evaluated the ultrastructural changes in dentin after treatment with the Nd:YAG laser and/or metal salt solutions and verified the presence of Sn++, Sr++, and F- in dentin structure.
Study Designs/Materials And Methods:
Sixty dentin disks were randomly divided into groups (n = 10): (I) control (no treatment), (II) Nd:YAG (1.5 W, 100 mJ, 15 Hz, 125 J/cm2), (III) 10% SnF2 aqueous solution for 30 minutes, (IV) Nd:YAG+10% SnF2 aqueous solution for 30 minutes, (V) 10% SrCl2 toothpaste for 30 minutes, (VI) Nd:YAG+10% SrCl2 toothpaste for 30 minutes. Then, all samples were prepared for scanning electron microscopy (SEM) and the samples from Groups I to IV for the energy dispersive X-ray microanalysis (EDX).
Results:
SEM evaluation revealed occluded dentinal tubules and a dentin surface altered by the laser irradiation. The EDX microanalysis revealed Sn++ at a depth of 250 microm in Group IV and not deeper than 100 microm in Group III. In Group V, Sr++ was not deeper than 50 microm, but it could be detected at a depth of 500 microm in Group VI. F- was found only in Group IV.
Conclusions:
Ultrastructural changes caused by laser irradiation can increase dentin uptake of Sn++, Sr++, and F-.
