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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
[Penicillium-inhibiting yeasts]
M R Benítez Ahrendts1, L Carrillo
1Facultad de Ciencias Agrarias, Universidad Nacional de Jujuy, Alberdi 47, 4600 SS Jujuy.
Abstract:
The objective of this work was to establish the in vitro and in vivo inhibition of post-harvest pathogenic moulds by yeasts in order to make a biocontrol product. Post-harvest pathogenic moulds Penicillium digitatum, P. italicum, P. ulaiense, Phyllosticta sp., Galactomyces geotrichum and yeasts belonging to genera Brettanomyces, Candida, Cryptococcus, Kloeckera, Pichia, Rhodotorula were isolated from citrus fruits. Some yeasts strains were also isolated from other sources. The yeasts were identified by their macro and micro-morphology and physiological tests. The in vitro and in vivo activities against P. digitatum or P. ulaiense were different. Candida cantarellii and one strain of Pichia subpelliculosa produced a significant reduction of the lesion area caused by the pathogenic moulds P. digitatum and P. ulaiense, and could be used in a biocontrol product formulation.
Insights
This study explored using yeasts to control post-harvest fruit molds. Certain yeast strains, Candida cantarellii and Pichia subpelliculosa, effectively reduced fungal lesions, showing potential for biocontrol products.
Area of Science:
- Agricultural Science
- Microbiology
- Food Science
Background:
- Post-harvest fruit spoilage is often caused by pathogenic moulds.
- Developing effective biocontrol agents is crucial for reducing agricultural losses and chemical treatments.
Purpose of the Study:
- To identify and evaluate yeasts for their potential to inhibit post-harvest pathogenic moulds on citrus fruits.
- To establish in vitro and in vivo efficacy of selected yeasts as biocontrol agents.
Main Methods:
- Isolation and identification of yeasts and pathogenic moulds from citrus fruits.
- Macro- and micro-morphological and physiological characterization of yeast strains.
- In vitro and in vivo assays to assess the inhibitory activity of yeasts against Penicillium digitatum and P. ulaiense.
Main Results:
- Various yeasts were isolated and identified, including Brettanomyces, Candida, Cryptococcus, Kloeckera, Pichia, and Rhodotorula.
- Significant differences were observed in the in vitro and in vivo activities of yeasts against target moulds.
- Candida cantarellii and a strain of Pichia subpelliculosa demonstrated significant reduction in lesion area caused by P. digitatum and P. ulaiense.
Conclusions:
- Candida cantarellii and Pichia subpelliculosa show promise as biocontrol agents against post-harvest citrus fruit moulds.
- These yeast strains could be formulated into effective biocontrol products to manage fungal spoilage.
- Further research is warranted to optimize formulation and application for commercial use.
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