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Updated: Jul 15, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Cofactor regeneration at the lab scale
1Biochemical Engineering Group, Department of Biochemical and Chemical Engineering, Universität Dortmund, 44221 Dortmund, Germany. wichmann@bci.uni-dortmund.de
Lab-scale coenzyme regeneration systems, particularly for NAD+/NADH and NADP+/NADPH, have advanced significantly. Various in situ methods, including enzymatic and electrochemical approaches, show promise for efficient biocatalysis.
Area of Science:
- Biotechnology
- Biocatalysis
- Enzyme Engineering
Background:
- Coenzymes like NAD+/NADH and NADP+/NADPH are crucial for redox reactions in biological systems.
- Efficient regeneration of these coenzymes is essential for cost-effective biocatalytic processes.
- Lab-scale advancements in coenzyme regeneration have been a focus for two decades.
Purpose of the Study:
- To review progress in lab-scale coenzyme regeneration systems.
- To highlight NAD+/NADH and NADP+/NADPH-dependent oxidoreductase reactions.
- To discuss various in situ regeneration strategies and enzyme reaction engineering.
Main Methods:
- Review of in situ regeneration systems: whole cell, enzymatic, electro-enzymatic, chemical, and photochemical.
- Analysis of efficiency and novelty of presented methods.
- Reporting on progress in enzyme reaction engineering.
Main Results:
- Significant progress in lab-scale coenzyme regeneration systems over the past 20 years.
- Focus on NAD+/NADH and NADP+/NADPH-dependent oxidoreductase reactions.
- Development and evaluation of diverse in situ regeneration techniques.
Conclusions:
- Various coenzyme regeneration systems have been developed and assessed for lab-scale applications.
- Enzymatic and electro-enzymatic methods show particular promise for efficient NAD+/NADH and NADP+/NADPH regeneration.
- Continued progress in enzyme reaction engineering is vital for optimizing these systems.
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