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Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic
Xiao-Nan Cui1, Jian-Wu Tang, Li Hou
1Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China.
Aim:
To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
Methods:
A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
Results:
Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
Conclusion:
SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes.
