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Related Experiment Videos

Monocyte activation on polyelectrolyte multilayers.

Jason J Hwang1, Sandra Jelacic, Newton T Samuel

  • 1Department of Bioengineering, University of Washington, Box 351721, Seattle, WA 98195-1721, USA.

Journal of Biomaterials Science. Polymer Edition
|March 30, 2005
PubMed
Summary
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Polyelectrolyte multilayer films with hyaluronic acid (HA) and poly-L-lysine (PLL) unexpectedly activated monocytes, causing cell death. Surface diffusion of PLL influenced monocyte adherence and activation, impacting biomaterial design strategies.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Polyelectrolyte multilayer (PEM) films are engineered coatings for biomaterials.
  • Hyaluronic acid (HA) and poly-L-lysine (PLL) are common PEM components.
  • Understanding cell interactions with PEM surfaces is crucial for biomaterial development.

Purpose of the Study:

  • To investigate primary human monocyte adherence and activation on HA-PLL PEM films.
  • To characterize the surface properties of PEM films and their effect on monocytes.
  • To assess the impact of surface chemistry on monocyte response, including cytokine production and cell viability.

Main Methods:

  • Sequential layer-by-layer deposition of HA-PLL multilayers.
  • Surface characterization using Surface Plasmon Resonance (SPR) and Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS).

Related Experiment Videos

  • Monocyte adhesion and viability assays (LDH, Live/Dead staining).
  • Monocyte activation measurement via Tumor Necrosis Factor-alpha (TNF-alpha) ELISA.

    • Main Results:

    • PEM films were successfully constructed with a defined thickness and polymer coverage.
    • ToF-SIMS revealed no significant surface chemistry differences between PLL- and HA-terminated surfaces.
    • Monocyte adhesion decreased, while cell death increased on both PEM surfaces compared to controls.
    • Monocyte activation (TNF-alpha production) was significantly higher (8-fold) on both PEM surfaces compared to tissue culture polystyrene (TCPS).

    Conclusions:

    • Diffusion of PLL into the surface layer of HA-terminated PEM films directed monocyte adherence and activation.
    • PEM surface composition significantly influences monocyte response, leading to activation and cell death.
    • The diffusion of components in PEMs must be considered in biomaterial coating design to predict and control cellular interactions.