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Updated: Aug 18, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Capillary zone electrophoresis separation of enantiomers of lisuride
Frantisek Kvasnicka1, Bohumír Bíba, Ladislav Cvak
1Institute of Chemical Technology, Department of Food Preservation and Meat Technology, Technickà 3, Prague 6, Czech Republic. kvasnicf@vscht.cz
Abstract:
Lisuride is an ergot alkaloid derivative with dopaminergic activity. It is used for treatment of Parkinsonism and some other diseases associated with high level of prolactine. Lisuride is a chiral compound derived from natural ergot alkaloids. A new capillary zone electrophoresis (CZE) method capable of separating the enantiomers of lisuride was developed. Using the optimized conditions (acidic electrolyte with the addition of gamma-cyclodextrin (gamma-CD)) as low as 0.02% of undesirable L-lisuride can be detected. Selected method characteristics, i.e., linearity (0-20 mg/l), precision (2.0% at 5 mg/I), and accuracy (101 +/- 4% at 5 mg/l) were evaluated. The optimized method was applied for the analysis of real batches of Lisuride hydrogenmaleate and Lisuride base manufactured by IVAX Pharmaceuticals. It was found that they contain less than 0.02% of undesirable L-enantiomer.
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