Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detecting microdomains in intact cell membranes.

B Christoffer Lagerholm1, Gabriel E Weinreb, Ken Jacobson

  • 1Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. lagerholm@med.unc.edu

Annual Review of Physical Chemistry
|March 31, 2005
PubMed
Summary

The existence of lipid rafts in cell membranes is debated. This review assesses experimental techniques for identifying these membrane microdomains in intact cells, highlighting their strengths and limitations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SNM1A is crucial for efficient repair of complex DNA breaks in human cells.

Nature communications·2024
Same author

Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cells.

JCI insight·2023
Same author

Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.

The Journal of cell biology·2022
Same author

Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Cell death & disease·2022
Same author

Improved and Sustained Graduate Programs Diversity Outcomes: a 10-year Analysis and Summary of the Brown University IMSD Program.

Journal for STEM education research·2021
Same author

FOXN1 forms higher-order nuclear condensates displaced by mutations causing immunodeficiency.

Science advances·2021

Area of Science:

  • Cell Biology
  • Membrane Biophysics

Background:

  • Current cell membrane models emphasize spatial heterogeneity and its relation to cell function.
  • Putative lipid rafts are proposed based on detergent insolubility and localization of signaling molecules in detergent-resistant membranes.
  • The in vivo existence of lipid rafts in intact cell membranes remains controversial due to inconclusive evidence.

Purpose of the Study:

  • To review experimental techniques for detecting lipid microdomains in intact cell membranes.
  • To assess the strengths and limitations of various methods for microdomain identification and characterization.

Main Methods:

  • Review of experimental techniques used or proposed for lipid microdomain detection.
  • Analysis of experimental results from different techniques.

Related Experiment Videos

  • Assessment of technique strengths and limitations for microdomain identification.

    • Main Results:

    • Various experimental techniques exist for investigating lipid microdomains.
    • Each technique has specific strengths and limitations for identifying and characterizing microdomains in intact cell membranes.
    • Evidence for lipid rafts in vivo remains inconclusive despite extensive research.

    Conclusions:

    • Further development and critical evaluation of experimental techniques are needed to definitively establish the in vivo existence and function of lipid rafts.
    • Understanding membrane heterogeneity is crucial for comprehending cell function.