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Published on: April 26, 2016
Application of immobilized enzyme reactor in on-line high performance liquid chromatography: a review
Anna Maria Girelli1, Enrico Mattei
1Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, P.le A. Moro 5, 00185 Roma, Italy. annamaria.girelli@uniroma1.it
Abstract:
This review summarizes all the research efforts in the last decade (1994-2003) that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC). All immobilization procedures including supports, kind of assembly into chromatographic system and methods are described. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. A brief survey of the main applications of IMER both as pre-column, post-column or column in the chemical, pharmaceutical, clinical and commodities fields is also reported.
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