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A sensitive liquid chromatography-tandem mass spectrometry method for the quantification of mometasone furoate in
Srikumar Sahasranaman1, Yufei Tang, Diana Biniasz
1Department of Pharmaceutics, College of Pharmacy, PO 100494, University of Florida, Gainesville, FL 32610, USA.
Abstract:
A robust, rapid, selective and sensitive liquid chromatography-negative atmospheric pressure chemical ionization (LC-(APCI(-))-MS-MS) method has been developed for the quantification of mometasone furoate (MF) in human plasma utilizing a solid-phase extraction clean-up step and 13C-fluticasone propionate as internal standard. The intra- and inter-day coefficients of variation were < or = 15% and the lower limit of quantification (LLOQ) was 15 pg/ml. This method is ideally suited for pharmacokinetic investigations of low MF levels following inhalation of MF.
