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Related Experiment Videos

Trypsin crystallization by membrane-based techniques.

Gianluca Di Profio1, Efrem Curcio, Enrico Drioli

  • 1Institute on Membrane Technology (ITM-CNR), c/o University of Calabria, Via P. Bucci, CUBO 17/C I-87030, Arcavacata di Rende (CS), Italy. g.diprofio@itm.cnr.it

Journal of Structural Biology
|March 31, 2005
PubMed
Summary

Membrane crystallization offers a novel method for growing protein crystals with controlled characteristics. This technique enhances crystallization kinetics and allows for precise modulation of crystal morphology for applications in structural biology and biotechnology.

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Area of Science:

  • Biotechnology
  • Structural Biology
  • Biochemistry

Background:

  • Growing protein crystals with controlled shape and size is challenging.
  • Membrane crystallization is a promising technique for enhancing protein crystallization kinetics.
  • This method can yield high-quality crystals without detrimental effects.

Purpose of the Study:

  • To investigate membrane crystallization for producing benzamidine inhibited trypsin from bovine pancreas (BPT) crystals.
  • To demonstrate control over BPT crystal morphology using membrane crystallization.
  • To explore the modulation of crystallization kinetics through process parameters.

Main Methods:

  • Studied membrane crystallization of BPT using ammonium sulphate as a precipitant.
  • Employed both static and forced solution flow configurations.

Related Experiment Videos

  • Investigated the impact of active membrane surface area and flow rates on crystallization.

    • Main Results:

    • BPT crystals were obtained within 24-48 hours in static configuration and 4-7 days in forced flow.
    • Crystallization kinetics were successfully modulated by controlling operative parameters.
    • Morphological characteristics of BPT crystals were influenced by membrane surface and flow rates.

    Conclusions:

    • Membrane crystallization provides a highly controllable method for growing protein and enzyme crystals.
    • This technique offers precise control over the final crystalline material properties.
    • Potential fundamental implications for structural biology and biotechnology applications.