Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging mass spectrometry: principles and potentials.

Pierre Chaurand1, Sarah A Schwartz, Michelle L Reyzer

  • 1Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University Medical Center, Nashville, Tennessee 37212, USA.

Toxicologic Pathology
|April 5, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B cell expression of an enzymatic intermediary in ether lipid biosynthesis promotes antibody responses and germinal center size.

eLife·2026
Same author

Advanced Dimensionality Reduction for Imaging Mass Spectrometry of Human Eye Tissue through Low-Rank Modeling with Sparse and Dense Residuals.

Analytical chemistry·2025
Same author

LRRK2 kinase activity regulates Parkinson's disease-relevant lipids at the lysosome.

Molecular neurodegeneration·2025
Same author

A lipid atlas of the human kidney.

Science advances·2025
Same author

Spatial characterization of RPE structure and lipids in the PEX1-p.Gly844Asp mouse model for Zellweger spectrum disorder.

Journal of lipid research·2025
Same author

<i>Helicobacter pylori</i> CagA and Cag type IV secretion system activity have key roles in triggering gastric transcriptional and proteomic alterations.

Infection and immunity·2025
Same journal

Comparison of Standard Clinical Pathology Parameter Values in Fasted and Fed Rats and Non-human Primates.

Toxicologic pathology·2026
Same journal

Time-Dependent Autolysis in Ex Vivo-Maintained Rat Tissue in Saline: A Descriptive Study and Practical Guidance for Histopathologic Assessment.

Toxicologic pathology·2026
Same journal

New Approach Methodologies (NAMs) for Carcinogenicity Evaluation.

Toxicologic pathology·2026
Same journal

2025 International Academy of Toxicologic Pathology (IATP) Satellite Symposium: Pathology Working Groups (PWGs) in Toxicologic Pathology.

Toxicologic pathology·2026
Same journal

Toxicologic Pathology Forum*: Opportunities and Challenges in the Use of Artificial Intelligence in Nonclinical Toxicologic Histopathology Evaluations.

Toxicologic pathology·2026
Same journal

New Modalities and Carcinogenicity Assessment.

Toxicologic pathology·2026
See all related articles

Direct tissue profiling using imaging mass spectrometry (MS) maps protein expression spatially. This advanced proteomic analysis aids in understanding disease states and predicting patient response to therapies.

Area of Science:

  • Proteomics
  • Biotechnology
  • Medical Diagnostics

Background:

  • Detailed mapping of protein expression within tissues is crucial for understanding disease mechanisms.
  • Spatial proteomic information can correlate protein levels with histological features and disease states.

Purpose of the Study:

  • To discuss the technology and methods of direct tissue profiling and imaging mass spectrometry (MS).
  • To highlight applications of MS in analyzing protein patterns in human tumors and their response to therapy.

Main Methods:

  • Direct tissue analysis using profiling and imaging mass spectrometry (MS).
  • Spatial mapping of protein expression across tissue sections.
  • Correlation of protein patterns with histological regions and disease states.

Related Experiment Videos

Main Results:

  • Profiling and imaging MS enable detailed spatial mapping of complex protein patterns in tissues.
  • These techniques have been applied to characterize human gliomas and lung cancers.
  • Demonstrated utility in assessing tumor response to therapeutics and correlating protein changes with drug therapy.

Conclusions:

  • Direct tissue profiling and imaging MS are powerful tools for spatial proteomic analysis.
  • Proteomic information derived from MS can aid in assessing disease progression and predicting patient treatment response.
  • MS applications include profiling human tumors, differentiating tumor grades, and monitoring drug effects.