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Related Experiment Videos

Antifreeze proteins modify the freezing process in planta.

Marilyn Griffith1, Chelsey Lumb, Steven B Wiseman

  • 1Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.

Plant Physiology
|April 5, 2005
PubMed
Summary

Winter rye antifreeze proteins (AFPs) do not protect cells directly but slow ice crystal growth and recrystallization, reducing freezing injury in plants. These AFPs are mobile and interact with ice, modifying the freezing process to enhance cold hardiness.

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Area of Science:

  • Plant Physiology
  • Cryobiology
  • Molecular Biology

Background:

  • Winter rye (Secale cereale L. cv Musketeer) accumulates antifreeze proteins (AFPs) in leaf and crown apoplast during cold acclimation.
  • AFPs are hypothesized to enhance freezing tolerance by modifying ice formation or acting as cryoprotectants.

Purpose of the Study:

  • To investigate the role of winter rye AFPs in plant survival at subzero temperatures.
  • To determine if AFPs modify the freezing process or provide cryoprotection.

Main Methods:

  • Assessed AFP mobility in guttate from cold-acclimated rye leaves.
  • Used infrared video thermography to observe freezing in winter rye leaves with and without ice nucleators.
  • Performed in vitro studies on ice recrystallization inhibition and ice migration.

Related Experiment Videos

  • Evaluated cryoprotective effects on lactate dehydrogenase activity and thylakoid structure.

    • Main Results:

    • Rye AFPs exhibited antifreeze activity, indicating mobility in solution.
    • AFPs lowered leaf freezing temperature by 0.3–1.2 °C in the presence of an ice nucleator.
    • In vitro, AFPs inhibited ice recrystallization and slowed ice migration.
    • AFPs did not confer specific cryoprotection to enzymes or thylakoid membranes.

    Conclusions:

    • Winter rye AFPs do not possess specific cryoprotective activity.
    • AFPs directly interact with ice in planta, reducing freezing injury by inhibiting ice growth and recrystallization.
    • The primary mechanism of AFPs in winter rye cold hardiness involves modifying the freezing process.