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Updated: Jul 11, 2026
Measuring and Modeling Contractile Drying in Human Stratum Corneum
Published on: March 1, 2017
Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates
F Dreher1, B S Modjtahedi, S P Modjtahedi
1Department of Dermatology, University of California, San Francisco, CA 94143-0989, USA.
Background/Aims:
Examination of stratum corneum (SC) content with tape stripping and a colorimetric method is increasingly used. We examined the possible use of microplates in tandem with a colorimetric method to examine SC removed with tape stripping. As a corollary to this examination, the homogeneity of tape strips was examined.
Method:
The commonly used Lowry assay was adapted for 96-well plates. Tapes were divided into four regions and sample disks of 5 mm diameter were taken from each and analyzed for SC mass using the adapted Lowry assay.
Results:
Homogeneity of SC removal over different areas across a tape strip is limited.
Conclusion:
Quantification of SC by means of a 96-well microplate-based colorimetric method is feasible and shortens the time of analysis. However, when using D-Squame tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.
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