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Published on: December 17, 2016
Efficiency of adenovirus-mediated gene transduction in heart grafts in rats
J Fujishiro1, H Kawana, S Inoue
1Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi, University of Tokyo, Tokyo, Japan.
Aim:
We determined the characteristics of transgene expression of heart grafts following ex vivo gene transfer using an adenovirus vector. Transgene expression was assessed periodically in the same animals by a non-invasive bioimaging system.
Methods:
Rat heterotopic heart transplantation was performed in a syngenic combination. We infused 1 x 10(9) plaque-forming units of adenovirus vectors containing firefly luciferase gene into the heart graft via the coronary artery, with preservation at 4 degrees C and transplanted into the cervix of the recipient. Transgene expression was periodically visualized and quantified by a noninvasive bioimaging system without sacrificing experimental animals.
Results:
Transgene expression in the graft peaked at day 7 and then fell gradually. Transgene expression was also observed in the recipient liver.
Conclusions:
We have determined the time course of transgene expression in the heart graft. This constitutes important information about ex vivo gene therapy for heart grafts.
