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Comet Assay as an Indirect Measure of Systemic Oxidative Stress
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Effect of cigarette smoking on DNA damage of human cumulus cells analyzed by comet assay

Ildikó Sinkó1, Mónika Mórocz, János Zádori

  • 1Center for Assisted Reproduction, Kaáli Institute, University of Szeged, Semmelweis u. 1., H-6725 Szeged, Hungary. sinko@nucleus.szbk.u-szeged.hu

Reproductive Toxicology (Elmsford, N.Y.)
|April 6, 2005
PubMed

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Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...