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[Study on fluorescence method of pefloxacin]
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 1, 1997
Summary
A new, highly sensitive fluorescence method was developed for analyzing Pefloxacin antibiotic. This method accurately quantifies Pefloxacin in biological samples like human serum and urine.
Area of Science:
- Analytical Chemistry
- Pharmacology
Context:
- Pefloxacin is an antibiotic requiring sensitive analytical methods for therapeutic drug monitoring.
- Existing analytical techniques may lack the required sensitivity or specificity for biological matrices.
Purpose:
- To develop and validate a sensitive fluorescence-based analytical method for Pefloxacin.
- To establish optimal excitation and emission wavelengths for Pefloxacin fluorescence detection.
Summary:
- A novel fluorescence method utilizing excitation at 282nm and emission at 440nm was developed for Pefloxacin analysis.
- The method demonstrates high sensitivity with a lower limit of detection and linear calibration curves from 1 x 10(-9) to 1 x 10(-6) mol/L (R²=0.9999).
- Accurate Pefloxacin quantification was achieved in human serum albumin (95% recovery) and urine (105% recovery).
Impact:
- Provides a sensitive and reliable tool for Pefloxacin quantification in biological fluids.
- Facilitates pharmacokinetic studies and therapeutic drug monitoring of Pefloxacin.
- Enables precise measurement of Pefloxacin concentrations, potentially improving patient treatment outcomes.