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Published on: November 11, 2022
[Studies on chromatography fingerprint of huangqi by HPLC]
Fangdi Hu1, Jianxiong Zhao, Shilan Feng
1Lanzhou Medical College, Lanzhou.
Objective:
To study on chromatography fingerprint(CFP) of Huangqi herbs.
Method:
HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution. CFP was referenced to formononetin, calycosin and then the contents of astragaloside IV, formononetin and calycosin was determinted.
Result:
26 common peaks were pointed out, their similarity of 12 samples were more than 90%.
Conclusion:
The handling of samples were simple. The study method on chromatography fingerprint of Huangqi were reliable. Good repetition and their similarity accorded with the demand on "the technology guide to experiment studies on chromatography fingerprint of injection of traditional Chinese medicine (TCM)", which was issued by the nation pharmacopoeial commission. The chromatography fingerprint and determination of index composition could be used to comprehensively control the quality of Huangqi.
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