Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maintenance of cochlear function with artificial oxygen carriers.

J Wada, S Paloheimo, I Thalmann

    The Laryngoscope
    |September 1, 1979
    PubMed
    Summary

    Vascular perfusion using FC 47 artificial blood successfully maintained cochlear function in guinea pigs for over 90 minutes. This method aids in studying cochlear biochemistry and pharmacology.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of gloved hand disinfection on hand hygiene before infection-prone procedures on a stem cell ward.

    The Journal of hospital infection·2019
    Same author

    Survival after neoadjuvant chemotherapy with or without bevacizumab or everolimus for HER2-negative primary breast cancer (GBG 44-GeparQuinto)†.

    Annals of oncology : official journal of the European Society for Medical Oncology·2014
    Same author

    Calibrated finger rub auditory screening test (CALFRAST).

    Neurology·2009
    Same author

    The Digital Cytocochleogram.

    Hearing research·2004
    Same author

    Expression of alpha and beta parvalbumin is differentially regulated in the rat organ of corti during development.

    Journal of neurobiology·2004
    Same author

    Proteomics and the inner ear.

    Disease markers·2002

    Area of Science:

    • Oto-rhino-laryngology
    • Neuroscience
    • Biochemistry

    Background:

    • Maintaining cochlear function during experimental procedures is crucial for research.
    • Vascular perfusion techniques are vital for studying inner ear physiology and pharmacology.

    Purpose of the Study:

    • To evaluate the efficacy of vascular perfusion with an FC 47-based blood substitute for maintaining cochlear function.
    • To assess the metabolic and structural integrity of the cochlea during perfusion.
    • To demonstrate the utility of this perfusion model for biochemical and pharmacological investigations.

    Main Methods:

    • Vascular perfusion of the guinea pig cochlea via the anterior inferior cerebellar artery.
    • Utilized a blood substitute containing the perfluorochemical FC 47 as an oxygen carrier.

    Related Experiment Videos

  • Measured cochlear microphonics and endolymphatic potential.
  • Assessed levels of ATP and 5' AMP in the stria vascularis and organ of Corti.
  • Examined the structural appearance of the cochlea post-perfusion.

    • Main Results:

    • Normal or near-normal cochlear microphonics and endolymphatic potential were maintained for over 90 minutes.
    • ATP and 5' AMP levels in the stria vascularis and organ of Corti were comparable to controls after 60 minutes of perfusion.
    • The organ of Corti appeared normal, but vacuoles were observed in the stria vascularis marginal cells.
    • Preliminary data on survival, revival times, and drug responses (furosemide, ouabain, mersalyl) were obtained.

    Conclusions:

    • Vascular perfusion with FC 47 artificial blood is a viable method for preserving cochlear function and metabolic state.
    • This technique provides a valuable model for in vivo studies of cochlear physiology, biochemistry, and pharmacology.
    • The observed vacuoles suggest potential limitations or areas for further investigation regarding FC 47 deposition.