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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
Published on: July 13, 2016
Polarised distribution of the RNA-binding protein Staufen in differentiated intestinal epithelial cells
Hannah Gautrey1, Josie McConnell, Judith Hall
1Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK.
Abstract:
mRNA localisation, as a mechanism for directing localised protein synthesis, plays a vital role in the functioning of certain cells, such as neurons and oocytes. Potentially this mechanism may also occur in polarised intestinal epithelial cells. Here we show that Staufen1(55), a protein involved in mRNA localisation and transport, is asymmetrically distributed in differentiated Caco-2 intestinal epithelial cells and partly co-localised with calnexin, a marker of the endoplasmic reticulum. The localisation to the apical region of the cell indicates that Staufen may be involved in localisation of transcripts to this domain.
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