Related Experiment Video
Updated: Aug 18, 2026
A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
Published on: August 14, 2013
Experimental observation of intraocular pressure changes during microkeratome suctioning in laser in situ
Hiroko Bissen-Miyajima1, Shintaro Suzuki, Yoshie Ohashi
1Department of Ophthalmology, Tokyo Dental College, Suidobashi Hospital, Tokyo, Japan. bissen@tdc.ac.jp
Purpose:
To observe changes in intraocular pressure (IOP), the manner in which the eye is suctioned, and the effect of the number of suction ports.
Setting:
Department of Ophthalmology, Tokyo Dental College, Suidobashi Hospital, Tokyo, Japan.
Methods:
Suction rings with single or dual ports were made of transparent acrylic acid resin to facilitate observation with a digital video camera. The IOP and the duration of the IOP increase (time to reach 90% of the maximum IOP) were measured in 6 porcine eyes with an intra-vein pressure-sensor catheter in the vitreous cavity. The IOP changes were recorded using a personal computer.
Results:
Suctioning with single- and dual-port suction rings was similar. The IOP increases with single- and dual-port suction rings were similar (99.1 mm Hg +/- 6.1 [SD] and 99.0 +/- 6.5 mm Hg, respectively) (P=.987). The duration of the IOP increase was also similar (4.21 +/- 0.24 seconds and 4.12 +/- 0.33 seconds, respectively) (P=.190).
Conclusions:
The number of ports did not affect suctioning and changes in IOP. This technique is useful in developing the ideal shape and setting of the suction ring.
