[Vital fluorochroming showing the effect of cyclophosphamide on the embryo]

Besides the usual methods, vital fluorochroming may globally show the extent of the cytotoxic effect of Cyclophosphamide (CP). By intravital treatment with highly diluted fluorochromes (acridine-orange, etc.) necrobiotic and dead cells, cell debris and phagosomes appear electively fluorescent. Glycosaminoglycans and various mucopolysaccharides show a somewhat weaker fluorescence. Autopods and the mesencephalon were selected for comparative assessment of the effect of other cytotoxic agents. Important results were also obtained by the repeated examination of blood (erythrocytes) and of hemopoetic foci. Cytotoxic effect within the embryo, teratogenesis and chemotherapeutic action are strongly correlated.