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Related Experiment Videos

Sterically stabilized liposomes. Reduction in electrophoretic mobility but not electrostatic surface potential.

M C Woodle1, L R Collins, E Sponsler

  • 1Liposome Technology, Inc., Menlo Park, California 94025.

Biophysical Journal
|April 1, 1992
PubMed
Summary

Polyethylene glycol-phosphatidylethanolamine (PEG-PE) in liposomes reduces electrophoretic mobility due to steric hindrance, not charge. This method accurately measures small mobilities, revealing PEG-PE

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Area of Science:

  • Colloid and Surface Science
  • Biophysical Chemistry
  • Materials Science

Background:

  • Liposomes are crucial drug delivery systems.
  • Surface charge influences liposome behavior and interactions.
  • Polyethylene glycol (PEG) modification is used to alter liposome properties.

Purpose of the Study:

  • To quantify the electrophoretic mobility of liposomes functionalized with polyethylene glycol-phosphatidylethanolamine (PEG-PE).
  • To investigate the impact of PEG-PE on liposome surface charge and mobility.
  • To elucidate the mechanism behind mobility reduction in PEG-PE modified liposomes.

Main Methods:

  • Doppler electrophoretic light scattering (DE LS) was employed to measure electrophoretic mobility.
  • Measurements were taken at multiple angles to minimize artifacts.

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  • Surface potential was assessed using an anionic fluorescent probe.

    • Main Results:

    • Liposomes with 5-10 mol% PEG-PE exhibited reduced electrophoretic mobility (-0.5 mu ms-1/Vcm-1) compared to phosphatidylglycerol (PG) liposomes (-2 mu ms-1/Vcm-1).
    • PEG-PE imparted a negative surface charge comparable to PG.
    • The reduction in mobility was attributed to steric effects of the PEG layer.

    Conclusions:

    • Electrophoretic light scattering is effective for measuring low mobilities.
    • The PEG layer on liposomes causes steric hindrance, reducing hydrodynamic drag.
    • An approximate PEG chain length of 50 Å was estimated for 2,000 molecular weight PEG.