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Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
Published on: January 20, 2014
A histomorphometric and cytogenetic study of testis from men 29-102 years old
Mbarka Dakouane1, Lucia Bicchieray, Marianne Bergere
1Laboratoire d'Histologie Embryologie, UFR des Saint Pères, Université Paris V et UFR Paris Ile de France Ouest, Université de Versailles-Saint Quentin, Paris. dakouane@hotmail.com
Objective:
To evaluate the influence of aging on testicular histology and the aneuploidy rate in testis postmeiotic cells.
Design:
Comparison between older men and younger men.
Setting:
Deceased donors and patients who requested assisted reproductive technology (ART).
Patient(S):
Thirty-six older men (61-102 years old) and 10 younger men (29-40 years old).
Intervention(S):
Testes were obtained from 35 deceased donors, and testicular biopsies were obtained from 11 patients who requested ART.
Main Outcome Measure(S):
Histomorphometry of testis and fluorescent in situ hybridization (FISH), with a three-probe set X, Y, and 18.
Result(S):
The histomorphometric study showed a thickening of the basal membrane when spermatogenesis was arrested. The number of germinal and Sertoli cells decreased as age increased. The rate of aneuploidy of postmeiotic cells was 1.1% for the control group, 1.29% for older subjects with preserved spermatogenesis, and 14.28% for the subjects with an arrested spermiogenesis. Only this last figure was higher than the control group.
Conclusion(S):
The rate of aneuploidy in older subjects (61-95 years old) with preserved spermatogenesis was not statistically different from that found in the control group; it was increased in older subjects with arrested spermatogenesis.
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