Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Non-destructive Raman analyses--polyacetylenes in plants.

Bernhard Schrader1, Hartwig Schulz, Malgorzata Baranska

  • 1Institut für Physikalische und Theoretische Chemie, Universität Duisburg-Essen, Soniusweg 20, D-45259 Essen, Germany. bernhard.schrader@uni-essen.de

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|April 12, 2005
PubMed
Summary

Fourier Transform Raman (FT-Raman) spectroscopy non-destructively identifies and quantifies plant acetylene compounds. This technique offers rapid analysis, replacing traditional methods and revealing compound distribution and changes during plant development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IR Map of the Human Cell.

Analytical chemistry·2026
Same author

Cell-in-water: complementary optical photothermal infrared and Raman microscopy for submicron chemical imaging of hydrated cells.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Temperature-induced spectral anomalies in doxorubicin-A Raman study.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Raman-guided analysis of drug response combined with chemometrics helps monitor the effect of ruxolitinib on acute lymphoblastic leukemia.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Intergenerational Transmission of Metabolic Changes in Oocytes From Aged Mice.

Aging cell·2026
Same author

Monitoring the Switching from Base-on to Base-off Forms of Vitamin B<sub>12</sub> by Natural and Magnetic Circular Dichroism Spectroscopies.

Analytical chemistry·2026

Area of Science:

  • Natural Product Chemistry
  • Spectroscopy
  • Plant Science

Background:

  • Ferdinand Bohlmann extensively studied plant-derived acetylene compounds.
  • Isolation, identification, and synthesis of these compounds were previously established.
  • Understanding the distribution and dynamics of these natural products is crucial.

Purpose of the Study:

  • To apply FT-Raman spectroscopy for non-destructive analysis of plant acetylene compounds.
  • To investigate the distribution and concentration of acetylene compounds within various plant parts.
  • To monitor changes in acetylene compound profiles during plant ontogenesis.

Main Methods:

  • Fourier Transform Raman (FT-Raman) spectroscopy was utilized with 1064 nm excitation.
  • Non-destructive analysis of plant samples was performed.

Related Experiment Videos

  • FT-Raman mapping was employed to visualize compound distribution.
  • A spectral library of known acetylene compounds, including synthesized standards, was used for identification.

    • Main Results:

    • FT-Raman spectroscopy successfully detected acetylene compounds in plants without interfering fluorescence.
    • Distinct acetylene compounds were identified with varying concentrations in different plant organs.
    • The distribution of these compounds across plant tissues was visualized using FT-Raman mapping.
    • Changes in compound profiles during plant development (ontogenesis) were successfully monitored.

    Conclusions:

    • FT-Raman spectroscopy is a rapid and effective method for analyzing plant acetylene compounds.
    • This technique bypasses time-consuming separation procedures and avoids artifacts.
    • FT-Raman spectroscopy enables detailed studies on the localization and temporal changes of natural products in plants.