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Effect of high density lipoproteins on protein expression in myoblast cell lines
Nikolina Babic1, Craig Beeson, Norman J Dovichi
1University of Washington, Department of Chemistry, P.O. Box 351700, Seattle, Washington 98195, USA.
Abstract:
We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 microg/mL HDL showed significant differences in the 7-10 pI region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.
