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Updated: Aug 18, 2026

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
[Lymphocyte subsets in patients with recurrent upper airway infections]
Ieva Bajoriūniene1, Daiva Urboniene, Genovaite Simoniene
1Department of Pulmonology and Immunology, Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 2, 50009 Kaunas, Lithuania. bajor@one.lt
Unlabelled:
The aim of our study was to evaluate the digressions of lymphocyte subsets in patients with recurrent upper airway infectious diseases.
Methods:
We studied 35 patients (mean of age 11.1+/-2.1 years) with recurrent upper airway infections. The first group consisted of patients, who had acute upper airway infections: rhinitis, pharyngitis, laryngitis and tracheitis more than 6 times per last year, sinusitis or otitis more than 4 times per last year. The control group comprised of 9 healthy subjects. Subsets of lymphocytes (CD3+, CD4+, CD8+, CD4+/CD8+, CD16+/56+ and CD19+) were detected by FACS Calibur cytometer.
Results:
We found a significantly lower count of CD4+ lymphocytes in the patients' group compared to the control group (37.5+/-1.2 vs 45.7+/-3.1% of total lymphocytes, p<0.01). We did not find any significant differences of other lymphocyte subsets between patients and control groups.
Conclusion:
We propose that patients with recurrent upper airway infections have alterations of the cellular immunity -- decreased amount of CD4+ lymphocytes.
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