[Study of the factors effecting surface-enhanced Raman scattering reporter-labeled immunogold colloids]

The applications of the high sensitivity of Surface-Enhanced Raman Spectroscopy (SERS) in labeled immunoassay have proved to be very useful. In the sandwich structure of "immobile antibody-antigen-labeled antibody", the authors prepared the labeled antibody by attaching gold nanoparticles, which was first labeled with molecule that had SERS signal, to the antibody. In this article, colloids were labeled with Raman reporter thiophenol through S-Au bond on gold and formed surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Then, goat anti-rat antibody was adsorbed onto the reporter-labeled gold colloids and formed reporter-labeled immunogold colloids. This paper studied factors affecting FT-SERS reporter-labeled immunogold colloids such as the sizes of gold particles, the amount of thiophenol in gold colloids and processing time, and whether goat anti-rat antibody would influence the SERS of reporter-labeled gold colloids.