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Assay for amoxycillin in urine using derivative spectrophotometry
A M el Walily1, M A el Sayed, M A Korany
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Alexandria, Egypt.
Journal of Clinical Pharmacy and Therapeutics
|April 1, 1992
Summary
Accurate amoxycillin (a penicillin antibiotic) quantification in urine is crucial. Three derivative spectrophotometric methods offer reliable, precise, and efficient analysis for clinical monitoring.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
- Clinical Chemistry
Background:
- Accurate quantification of amoxycillin in biological samples is essential for therapeutic drug monitoring.
- Existing analytical methods may have limitations in sensitivity or specificity for urine samples.
- Derivative spectrophotometry offers potential for improved resolution and quantification of drug analytes.
Purpose of the Study:
- To develop and validate three derivative spectrophotometric methods for the quantitative assay of amoxycillin in human urine.
- To evaluate the linearity, recovery, and precision of the proposed methods.
- To provide a reliable analytical tool for assessing amoxycillin levels in patients.
Main Methods:
- Development of three spectrophotometric methods utilizing amplitude measurements of second derivative (D2), first derivative difference (ΔD1), and second derivative difference (ΔD2) curves.
- Linear correlation established between derivative curve amplitudes and amoxycillin concentration.
- Validation of methods using urine specimens to determine recovery and precision.
Main Results:
- All three derivative spectrophotometric methods demonstrated a linear relationship with amoxycillin concentration within the range of 0.2-1.0 mg%.
- Negligible intercepts were observed, indicating high accuracy.
- An absolute recovery of 98% was achieved from urine specimens.
- Satisfactory within-day and between-day precision was obtained.
Conclusions:
- The developed derivative spectrophotometric methods provide a sensitive, accurate, and precise approach for amoxycillin determination in urine.
- These methods are suitable for routine clinical laboratory analysis and therapeutic drug monitoring.
- Derivative spectrophotometry offers an advantageous alternative for amoxycillin assay in complex biological matrices.