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Related Experiment Videos

Skin testing versus radioallergosorbent testing for indoor allergens.

Birjis Chinoy1, Edgar Yee, Sami L Bahna

  • 1Allergy and Immunology Section, Louisiana State University Health Sciences Center; Shreveport, Louisiana, USA. sbahna@lsuhsc.edu.

Clinical and Molecular Allergy : CMA
|April 19, 2005
PubMed
Summary

Skin testing (ST) is more sensitive than serum specific IgE tests (RAST) for indoor allergens like D. farinae, cockroach, cat, and dog. ST is superior for detecting sensitization to cat and dog allergens, even without reported exposure.

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Area of Science:

  • Allergy and Immunology
  • Diagnostic Testing
  • Respiratory Medicine

Background:

  • Skin testing (ST) and serum specific IgE measurement are common allergy screening methods.
  • Sensitivity and specificity of allergy tests can vary by allergen and manifestation.
  • Comparison of ST and serum specific IgE (RAST) for indoor allergens in respiratory allergy patients was needed.

Purpose of the Study:

  • To compare the reactivity of skin testing (ST) with serum specific IgE antibodies (RAST) to common indoor allergens.
  • To evaluate the diagnostic performance of ST versus RAST in patients with allergic rhinitis and/or asthma.

Main Methods:

  • 118 patients with allergic rhinitis/asthma underwent percutaneous and intradermal skin testing.
  • Serum samples were tested for specific IgE antibodies using the radioallergosorbent test (RAST).

Related Experiment Videos

  • Allergens included D. farinae, cockroach, cat epithelium, and dog epidermal; results were scored and compared.

    • Main Results:

    • Skin testing (ST) demonstrated higher sensitivity than RAST for all four indoor allergens.
    • ST was superior to RAST in detecting sensitization to cat and dog allergens, irrespective of reported exposure.
    • Agreement between ST and RAST ranged from 52.2% to 62.2%; correlation was weak to moderate (r = 0.24–0.54).

    Conclusions:

    • Skin testing (ST) is more sensitive than RAST for diagnosing sensitization to common indoor allergens.
    • ST is particularly effective for identifying sensitization to cat and dog allergens.
    • Positive results from ST and RAST showed poor correlation in score, suggesting complementary diagnostic value.