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[Study on inhibitory effect of matrine on cyclooxygenase-2 expression in colon cancer HT-29 cell line]
Jian Huang1, Ming-jie Zhang, Fu-ming Qiu
1Department of Oncology, The Second Hospital Affiliated to Medical College of Zhejiang University, Hangzhou. hjys@zju.edu.cn
Objective:
To explore the effect of matrine on cyclooxygenase-2 (COX-2) expression in colon cancer HT-29 cell line at the level of gene and protein.
Methods:
Levels of mRNA and protein expression of COX-2, and its synthesized product prostaglandin E2 (PGE2) of colon cancer HT-29 cell line were detected by RT-PCR, Western-blot, ELISA respectively before and after treatment of matrine in different concentrations.
Results:
Matrine had inhibitory effect on the mRNA and protein expression of COX-2, and synthesis of PGE2 in colon cancer HT-29 cell line, but had no effect on COX-1. When HT-29 cell line was treated with 2.0 mg/ml of matrine, the inhibitory rate on COX-2 mRNA expression were 100% at 6 hrs and 9 hrs after treatment; the inhibitory rate on PGE2 synthesis was 63.8 % at 9 hrs after treatment; and that on COX-2 protein expression was 48% and 100% 12 hrs and 24 hrs after treatment, respectively.
Conclusion:
Matrine has selective inhibitory effect on gene transcription, protein expression and functional activity of COX-2 in HT-29 cell line, which is time-dependent and concentration-dependent within certain range of concentration and acting time.
Insights
Matrine selectively inhibits cyclooxygenase-2 (COX-2) gene and protein expression in colon cancer cells. This natural compound effectively reduces prostaglandin E2 synthesis, offering a potential therapeutic strategy.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Cyclooxygenase-2 (COX-2) is implicated in colon cancer development and progression.
- Targeting COX-2 is a strategy for colon cancer therapy.
- Matrine is a natural compound with potential anti-cancer properties.
Purpose of the Study:
- To investigate the effect of matrine on cyclooxygenase-2 (COX-2) expression at the gene and protein levels in the HT-29 colon cancer cell line.
- To determine if matrine affects COX-1 expression.
- To assess the impact of matrine on prostaglandin E2 (PGE2) synthesis.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) to measure COX-2 mRNA levels.
- Western-blot assay to quantify COX-2 protein expression.
- Enzyme-linked immunosorbent assay (ELISA) to detect PGE2 synthesis.
Main Results:
- Matrine demonstrated a dose- and time-dependent inhibitory effect on COX-2 mRNA and protein expression in HT-29 cells.
- Matrine significantly reduced PGE2 synthesis in a time-dependent manner.
- No significant effect of matrine was observed on COX-1 expression.
Conclusions:
- Matrine exhibits selective inhibition of COX-2 gene transcription, protein expression, and functional activity in the HT-29 colon cancer cell line.
- The inhibitory effects are dependent on both concentration and duration of exposure within tested ranges.
- Matrine represents a potential therapeutic agent for colon cancer by selectively targeting COX-2.
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